Perbandingan Hasil Pemeriksaan Antinuclear Antibodies dengan Metode Imunofluoresens dan Metode Elisa pada Penderita Tersangka Systemic Lupus Erythematosus di Rumah Sakit Dokter Hasan Sadikin Bandung

Penny Setyawati Martioso


Systemic lupus erythematosus (SLE) is characterized by the presence of anti-nuclear antibodies (ANAs). The identification of ANA has considerable importance in diagnosis and management of patients with autoimmune diseases. The aims of this study were to compare the validity of ANA in the screening of SLE by estimated sensitivity, specificity, positive predictive values (PPV) and negative predictive values (NPV), and coefficient of agreement between FANA and ANA-ELISA.

Sixty female outpatients suspected SLE, who fulfilled 2 or 3 of 11 criterias American College of Rheumatology (ACR) 1997, aged between 15-45 years old, from the Department of Internal Medicine Dokter Hasan Sadikin General Hospital (RSHS) Bandung were selected for this study. In this study, antibodies to nuclear antigens in all patients serum specimens were identified by qualitative FANA and ANA-ELISA. The data was analyzed by diagnostic test.

The diagnosis of SLE was establised in 40 patients, 39 had FANA positive, 38 had ANA-ELISA positive. The validity of FANA vs ANA-ELISA were: Sensitivity 97,5% vs 95%; Specificity 20% vs 45%; PPV 70,9% vs 77,5%; NPV 80% vs 80,8%; 0,215467 vs 0,449409.

Conclusions: The Sensitivity of the two methods were high, but ANA-ELISA was more spesific and significant (k: 0,449409; p: 0.0001670) in diagnosis of patients with Systemic lupus erythematosus.


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